Panbio Pan-E Dengue Early ELISA Kit and Dengue NS1 Antigen

Dengue is an arbovirus belonging to the genus of Flavivirus in the family Flaviviridae.It is the aetiological agent that cause dengue fever (DF) and dengue hemorrhagic fever (DHF).Currently, up to 2.5 billion people living in the tropical belt of the world are at risk of the infection.Annually, 50 to 100 million DF were reported with 500,000 DHF resulting in 25,000 deaths and a high proportion were children.Significant number of patients suspected of having dengue infection had IgM antibody detected in their serum.In 2006, Sabah and Sarawak had reported DF and DHF with significant number of deaths. Detection of dengue antibodies are among many laboratort tests routinely performed to confirm dengue in patients but these antibodies were often undetectable in the early phase of the infection.NS1, a -50-kilodalton glycoprotein, is one of seven non-structural (NS) protein genes of the dengue virus.

Preliminary studies have shown its association in viral RNA replication.Dengue NS1 antigen has been reported to be detected in serum from day 1 after onset of fever. It has been shown that NS1 antigen could be used as a suitable marker of dengue virus infection.Early definitive diagnosis is essential for the successful timely management of the infection.Performing preliminary evaluation of panbio pan-E Early Dengue ELISA kit on patient serum suspected of dengue is recommended and compared it with the panbio Dengue Duo Cassette, panbio Dengue IgM Capture ELISA and Dengue real-time polymerase chain reaction(RT-PCR).In one study reported that panbio pan-E Dengue Early ELISA was able to detect NS1 antigen when panbio Dengue IgM Capture ELISA was still negative in the early phase of the disease.