Blood Collection from Orbital Sinus in Mice

• Should apply anesthetic before blood withdraw
• A convenience and easy apply method for blood collection in mouse
• Collect amount up to 0.5 ml

Tools for Blood Collection from Orbital Sinus in Mice

• 75% alcohol cotton ball for surface disinfection
• Hypnorm for general anesthetic
• 27 G needle with 1 ml syringe for injection
• Glass capillary tube and vial for blood collection

Anesthetize a mouse by intraperitoneal injection of Hypnorm

Use a sharp end glass capillary tube to penetrate the orbital conjunctiva and rupture the orbital sinus

Collect blood with a vial