Blood Collection from Tail Vein in Mice

• For collection of small amount of blood (Approximate 0.1 ml)

Tools for Blood Collection from Tail

• 75% alcohol cotton ball for surface disinfection
• Small plastic bottle with 1/2 cm diameter holes in both ends as mouse restrainer
• Scissors
• Pipetteman and tips
• A vial for blood collection

Placing a mouse on a cage lid and grasping the loose skin behind the ears by the thumb and forefinger

Push the mouse into the restrainer

Leave the tail of the mouse outside the cover of the restrainer

Amputate the tip of the mouse tail by scissors

Massage the tail and collect blood by pipetteman