Tuesday, March 30, 2010

The use of fabric softener ‘Softlan’ as a tissue softener prior to paraffin wax sectioning



Fabric softener such as ‘Softlan’ has been used before in the reconstitution of mummified or desiccated tissue prior to paraffin wax sectioning for light microscopical examination. A method in which a fabric softener ‘ Softlan’ is used to soften hard tissue. The paraffin tissue blocks are soaked in diluted Softlan (1 part into 5 parts of tap water) at room temperature for five minutes before sectioning.

A comparison of results obtained by this method is made with those obtained by soaking overnight in fabric softener, placing in decalcifying solution Shadon TBD-1 Rapid Decalcifier for surface decalcification, and without treatment at all. Section obtained were found to be better and more complete with no effects on the nuclear and cytoplasmic staining

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