ABSTRACT
This study or experiment
investigates the Dose-Response relationship, focusing on the Effective Dose
(ED50) and Lethal Dose (LD50) from toxicology point of view. The major aim of
this experiment is to gain knowledge on how to estimate the effective dose that
will be done by in vivo toxicity testing. Secondly is to determine the
effective dose (ED50). Thirdly is to gain knowledge on how dose toxic effect
will be obtained by in vivo toxicity testing and finally, to determine the
median lethal dose (LD50). Approach that was used was by injecting a certain
type of drug intra-peritoneally to a number of test animals which for this
particular experiment are either rats or mice used and after that, the effect
was observed. The result obtained is assessed based on the Reed-Muench Method
(1938). The result obtained was, for ED50 was 20mg/kg while LD50 was 120 mg/kg meaning
that at 20 mg/kg, 50% of a group of test animals exposed to the drugs was
affected and also at 120mg/kg cause the death of 50% of a group of test animal.
Therefore the aim which was to estimate the effective dose and lethal dose was
achieved.
INTRODUCTION
In the field of
toxicology, the most essential concept is the dose-response relationship. Generally,
it shows direct proportion between the dose and the response whereby increase
dosage will cause increase effect and vice versa. From practical point of view,
two types of dose-response relationships
exist. First and foremost is the individual/graded dose-response relationship, also
recognized as the response of individual organism to varying doses of chemical
or graded due to the fact that the measured effect is continuous over a range
of doses. Secondly, acid or sodium salt. The
free acid is a fine white crystalline powder that is only slightly soluble in
water and ethanol. The salt is a white crystalline powder or granules and is
very soluble in water and ethanol. Pentobarbital is a popular short-acting
barbiturate derivative used primarily as a sedative. It is also used to reduce
intracranial pressure and lower
cerebral oxygen demand in patients with severe head trauma, Reye’s syndrome, or
anoxic brain damage. It is available alone or with other drugs in 15-200 mg
amounts for oral, intramuscular or rectal administration. (Baselt et al.1982)
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