Wednesday, September 17, 2008

Procedure for Leishman Staining and Differential WBC Count

Procedure of Leishman Staining

  • Let thin blood film to air dry
  • Then flood the slide with Leishman stain solution
  • Wait for 8 minutes. Then flood off with distilled water in 2 – 3 seconds. Longer washing will remove stain
  • Wait for 20 minutes. Observe the appearance of metallic cum that float on the surface of the slide.
  • Then wash with tap water. Stand in a rack to drain and air dry.

Perform differential white blood cells count

  • Using a low-power lens (10 x objectives) on the microscope, check the approximate differential cell distribution.
  • Using the 40x objective high-power lens, perform a 200 cell differential count.
  • 100x objective oil immersion lens should be used for examining fine intracellular details of the cells.
  • Move the slide along the stage of the microscope in a broad battlement track running transversely across the body of the film, avoiding the edges completely.
  • Record the numbers of each type of white cell. Calculate the percentage of each of the five basic leucocytes.


Anonymous said...

thankssss soooooo much

Anonymous said...

The procedure steps given here are incomplete. No one could possibly stain a thin blood film following the procedure given in this blog. I am sorry that I thought someone must indicate this so that another beginner will not be deceived by the procedure given in the blog. Thank you!

Anonymous said...