Fabric softener such as ‘Softlan’ has been used before in the reconstitution of mummified or desiccated tissue prior to paraffin wax sectioning for light microscopical examination. A method in which a fabric softener ‘ Softlan’ is used to soften hard tissue. The paraffin tissue blocks are soaked in diluted Softlan (1 part into 5 parts of tap water) at room temperature for five minutes before sectioning.
A comparison of results obtained by this method is made with those obtained by soaking overnight in fabric softener, placing in decalcifying solution Shadon TBD-1 Rapid Decalcifier for surface decalcification, and without treatment at all. Section obtained were found to be better and more complete with no effects on the nuclear and cytoplasmic staining
A comparison of results obtained by this method is made with those obtained by soaking overnight in fabric softener, placing in decalcifying solution Shadon TBD-1 Rapid Decalcifier for surface decalcification, and without treatment at all. Section obtained were found to be better and more complete with no effects on the nuclear and cytoplasmic staining
