Product Overview - Laboratory Microscope

Product Name: Laboratory Microscope

Product ID: MT4000

Description:

The Meiji MT4000 series biological laboratory microscopes include the advanced Infinity Corrected Optical System. The MT4000 series includes:

* Objective Lenses: DIN Infinity Corrected Optical System (ICOS) Plan brightfield 4x, 10x, s40x and s100x oil mounted in smooth-operating, ball bearing mounted, quintuple nosepiece.
* Viewing Head: Siedentopf-type binocular head or trinocular head for camera integration. Each head has the eyetubes inclined at 30° with the left eyetube having graduated diopter settings. The interpupillary distance is adjustable between 53mm - 75mm. An 80/20 beamsplitter for the trinocular tube can be engaged for photo work. (100% to eyetubes or 80% to phototube & 20% to the eyetube.)
* Eyepieces: 10x Widefield High Eyepoint eyepieces F.N. 20 are standard with a 19mm reticle mount. 15x and 20x eyepieces are available as an option. Also avilable - Widefield High Eyepoint 10x focusable eyepiece that accept 25mm reticles.
* Total Magnification: 40x, 100x, 400x and 1000x.
* Specimen Stage: Ceramic coated right-handed (left-handed avilable upon request), flat top stage measures 171mm x 115mm and travels 78mm(X) x 52mm(Y). Ergonomically positioned coaxial drop down controls.
* Illumination: Powerful white LED or 30 watt halogen illumination provides enhanced image quality and brightness for the observation of specimens and photomicroscopy.
* Light Focusing: Abbe 1.25 NA Condenser in quick change dovetail mount.
* Size & Weight:
* Binocular - 390mm (15.6") D x 410 (16.1") H x 210mm (8.3") W, 8kg (17.6 lbs).
* Trinocular - 390mm (15.6") D x 465 (18.3") H x 210mm (8.3") W, 8.7kg (19.2 lbs).

Introduction to Microscope

A microscope is an instrument for viewing objects that are too small to be seen by the naked or unaided eye. The science of investigating small objects using such an instrument is called microscopy. The term microscopic means minute or very small, not visible with the eye unless aided by a microscope.

Microscope Operating Guide - Part I

• Make sure the microscope is placed on a flat surface.
• For first time use prepare a slide with a mark made by marker crayon or a sharpie marker. You may use this mark to learn to focus the microscope.

Turn the revolving nosepiece to engage the 10X objective. Make sure that the revolving nosepiece stops with an audible click.

Lower the stage using the coarse adjustment and gently place a prepared slide on the stage in the specimen holder clips. The specimen holder clips are spring loaded and if forcibly released may break the slide.

Turn to 10x objective

Microscope Operating Guide - Part II

Microscope X Adjustment

Turn the X-axis and Y-axis adjustment knobs to move the mark on the slide into the light path. Do not move the stage manually without the adjustment knobs. There are limits to how far you can turn the knobs. Do not force the knobs.

Microscope Y Adjustment

Switch the main switch to ON and adjust the brightness with the light intensity knob

Microscope Main Switch On

Microscope Light Intensity Adjustment

Looking through the right eyepiece with your right eye, turn the coarse adjustment knob to bring the specimen into focus.When you have optimized the focus with the coarse adjustment controls, use the fine adjustment knob to improve the focus.

Microscope Coarse Adjustment

Microscope Operating Guide - Part III

Looking through the left eyepiece with you left eye, turn the diopter adjustment ring to focus the specimen.

Adjust the interpupillary distance between the eyepieces. Look through the eyepieces and manually adjust the space between the eyepieces until the left and right fields of view coincide completely.

Microscope Interpupillary Adjustment

Engage the objective to be used for observation by turning the revolving nosepiece. Refocus by rotating the coarse adjustment knob to reach the pre-focusing position, this is limited by the engaged pre-focusing lever. Make fine adjustments with the fine adjustment knob. Always focus "up", moving the objective away from the slide.

Engage the objective

Microscope Operating Guide - Part IV

Adjust the light intensity to the desired level, and then readjust the fine focus.

Microscope Light Intensity Adjustment

Adjust the field iris diaphragm

Field Iris Diaphragm Adjustment

Adjust the aperture iris diaphragm. The iris aperture diaphragm controls the cone of light that reaches the objectives and therefore controls the numerical aperture. Matching the numerical aperture of the illumination system (given on the iris diaphragm) with that of the objective provides better image resolution and contrast, and also increases the depth of focus.

Aperture Iris Diaphragm Adjustment

OIL EMMERSION

• The presence of oil between the objective and the specimen decreases the diffraction of light that as it passes through the objective and this is important for resolution of objects at 1000X (100X objective and 10X eye piece). To use the 100X objective, also called the oil immersion objective, follow the instructions given above and:
• Place a drop of immersion oil on the slide in the viewing area.
• Turn the revolving nosepiece to engage the oil immersion objective.
• Use the fine adjustment control to focus the image.
• The oil on the specimen must be free of bubbles or the image is impaired. To remove bubbles, slightly move the oil immersion lens back and forth over the specimen.
• It is very important that all oil be cleaned off the lens before storing the microscope.

Clean Microscope

CARE OF THE MICROSCOPE

Handling the microscope

• To carry a microscope: first clear your desk to receive the microscope, then grasp its arm firmly, lift and support under the base with other hand, set on a cleared desk. Remove and store its dust cover in cabinet under desk. Unwrap power cord, loop once around gas outlet at rear of desk, plug into electrical outlet in front of desk.
• Clean the lenses: use ONLY lens paper. Polish the objectives and oculars: breathe on them lightly for moisture.
• Always begin slide set-up with the stage lowered and the lowest power objective (4x) in place.
• Focus initially only by LOWERING the stage to the focal point using the coarse focus.
• Use only the fine focus with higher power objectives. Make only minor changes in focus when necessary with the fine focus knob. If you totally lose focus, return to a lower power objective to find the focal point. Do not use the 100x objective unless you have received specific instructions on its use.

Cleaning the microscope

• Don’t let the microscope get too dirty – always use the dust cover when not in use.
• To clean the eyepiece – use a high quality lens paper. First brush any visible dust from the lens, and then wipe the lens. Do not use facial tissues, they are made from ground up wood fibers and could damage the lenses.
• To clean the objective lenses – use a fresh piece of the lens paper each time so that you don't transfer dust from one lens to another.
• Use lens paper on all glass parts of the microscope.
• A cotton swab (Q-Tip) can be used in place of lens paper.